**The title, authors, and abstract for this completion report are provided below.  For a copy of the completion report, please contact the GLFC via e-mail or via telephone at 734-662-3209**

STRUCTURE DETERMINATION OF THE PRIMARY COMPONENTS OF THE SEA LAMPREY MIGRATORY PHEROMONE AND ELUCIDATION OF THEIR BIOLOGICAL IMPORTANCE

P.W. Sorensen¹ & T.R. Hoye²

¹ Department of Fisheries, Wildlife, and Conservation Biology
University of Minnesota
1980 Folwell Ave.
St. Paul, MN 55108

²Department of Chemistry
University of Minnesota
207 Pleasant St. SE
Minneapolis, MN 55455

Abstract

Three major components of the sea lamprey migratory pheromone have been identified. The most important compound contains an unprecedented 1-(3-aminopropyl)pyrrolidin-2-one subunit and is related to squalamine, an antibiotic produced by sharks. It is detected by the lamprey olfactory system at a concentration of 10-13Molar, a concentration at which it also attracts adult lamprey and that is a possible record for potency of a fish attractant. The structure has been synthesized and a behavioral experiment has confirmed activity. We have named this structure petromyzonamine disulfate (PADS). The second component is another new sulfated steroid. It also has two sulfate groups, and we have named it petromyzosterol disulfate (PSDS). It is detected by the lamprey olfactory system at a concentration of 10-12Molar and attracts migratory sea lamprey at a concentration of 10-11Molar. The third is petromyzonol sulfate (PS), a known lamprey-specific bile acid derivative. It is only weakly attractive at a concentration of 10-11Molar and appears to be a relatively minor component of the cue. Behavior experiments have shown that all three components contribute to the unique potency of the mixture; no single component is as attractive as the entire mixture, even when tested at supra-normal concentrations. This mixture is the first migratory pheromone identified in a vertebrate and should prove extremely useful in sea lamprey control.