**The title, authors, and abstract for this completion report are provided below.  For a copy of the completion report, please contact the GLFC via e-mail or via telephone at 734-662-3209**

Management-scale tests of a synthesized pheromone, 3kPZS, to influence stream selection of adult sea lampreys

Nicholas Johnson², Michael Siefkes³, Michael Wagner⁴, Weiming Li⁴, Michael Twohey⁵

² U. S. Geological Survey, Great Lakes Science Center, Hammond Bay Biological Station, 11188 Ray Road, Millersburg, MI 49759

³ Great Lakes Fishery Commission, 2100 Commonwealth Blvd., Suite 100, Ann Arbor, MI 48105

⁴ Michigan State University, Department of Fisheries and Wildlife, 13 Natural Resources Building, East Lansing, MI 48824

⁵ US Fish and Wildlife Service, Marquette Biological Station, 3090 Wright St., Marquette, MI 49855

December 2011

Abstract

Increased use of alternative controls in the sea lamprey (Petromyzon marinus) management program is an important milestone of the Great Lakes Fishery Commission vision. We tested the hypothesis that adult sea lamprey stream selection is influenced by the concentration of a synthesized sea lamprey pheromone component, 7a, 12a, 24-trihydroxy-5a-cholan-3-one 24-sulfate (3kPZS), in the stream plume emanating into the lake. 3kPZS was applied continuously to the mouths of the Misery and Rock Rivers from 01Mar10 to 23June10 to achieve a final in-stream concentration of 10^-12 Molar. Application of 3kPZS did not increase the percentage of the lake-wide abundance of sea lampreys in Lake Superior entering the Misery or Rock Rivers to the levels observed when the streams were infested with larval sea lampreys. The average percentage of lake-wide abundance migrating up the Misery River when infested was 2.81%, when not infested was 0.62%, and when 3kPZS was applied to the Misery River mouth was 0.58%. The average percentage of lake-wide abundance migrating up the Rock River when infested was 5.03%, when not infested was 1.65%, and when 3kPZS was applied to the Rock River mouth was 0.94%. Application of 3kPZS did not decrease the percentage of the lake-wide abundance of sea lampreys in Lake Superior entering streams neighboring the Misery and Rock Rivers. It is concluded that 3kPZS only modifies adult sea lamprey behavior in-streams and does not induce behavioral responses in adult sea lampreys in the lake. Given the results, experimental applications of 3kPZS to traps in 20 streams throughout the Great Lakes basin in 2010 and 2011 have not biased results of the regression model used to estimate adult sea lamprey abundance in un-trapped streams.