**ABSTRACT NOT FOR CITATION WITHOUT AUTHOR PERMISSION. The title, authors, and abstract for this completion report are provided below. For a copy of the full completion report, please contact the author via e-mail at liweim@msu.edu Questions? Contact the GLFC via email at slrp@glfc.org or via telephone at 734-662-3209.**
ELUCIDATION OF THE TFM METABOLISMS IN
SEA LAMPREY AND NON-TARGET FISH
Belinda
Huerta 2, Yu-Wen Chung-Davidson2, Ugo Bussy2, Ke Li2, Weiming Li2
2Department of Fisheries & Wildlife, Michigan State
University, East Lansing, MI 48824
December 2018
ABSTRACT:
TFM
metabolism in vivo was
studied in sea lamprey and non-target species, which allowed the
description and synthesis of several novel metabolites. Accordingly, a
quantitative method was developed for the assessment
of TFM and its metabolites in different matrices, including fish liver tissue
and water samples. Among the novel metabolites described, the presence of nitro
reductive metabolites as a consequence of the
reduction of TFM was hypothesized to be a determining factor
for sea lamprey sensitivity to this lampricide. Mitochondrial
oxidative phosphorylation in sea lamprey was studied
when exposed to both TFM and amino TFM, as well as their non-fluorinated
counterparts in order to determine if reductive metabolites could also uncouple
the ATP synthase or affect other complexes in the electron transfer chain to
the same degree of TFM. Amino TFM was found to affect
mitochondrial respiration, although at a higher concentration than TFM, which
suggests a lower toxicological effect than the parent compound.