**ABSTRACT
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abstract for this completion report are provided below. For a copy
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2Department of Fisheries &
Wildlife, Michigan State University, East Lansing, MI 48824
March 2019
ABSTRACT:
This project encompasses
a series of chemical-related endeavors to facilitate the study of sea lamprey
pheromones and the TFM metabolism in sea lamprey. Firstly, several analytical
methods were developed for the quantification of small
biomolecules (estrogens, thyroid hormones, steroids, and bile acids). Routine
analysis of these compounds have been constantly performed
in the funding period to study reproduction and migration cues in this species.
Additionally, TFM metabolism in vivo was studied in sea lamprey and non-target species. Several
novel metabolites were described and synthesized. A
quantitative method was developed for the assessment
of TFM and its metabolites in different matrices. Among these novel
metabolites, the reduction of TFM and subsequent presence of nitro reductive
metabolites were deemed as a determining factor for sea lamprey sensitivity to this
lampricide.